Pedro Delgado, the defending champion, missed his start time with 2:40 at the prologue at the start of the 1989 Tour de France. Delgado covered the distance only 14 seconds slower than Erik Breukink, the stage winner. But adding the 2 minutes and 40 seconds at his time, he became the only defending champion to begin the race in last place, 2:54 behind Breukink.
Greg LeMond surprised by placing fourth in the opening prologue. Laurent Fignon was second, Sean Kelly third with all three finishing six seconds behind the winner on the day, Erik Breukink. Delgado ended the first day of the race placed last of the 198 riders.